(Database for the nematode branch of
Assembling the Tree Of Life
)
Methods and Protocols
Common Protocols
DMSO/EDTA/NaCl protocol (
html file
)
18s Protocols
1
2
3
Extraction of Genomic DNA (
pdf file
) (
html file
)
PCR Amplifications by Locus (
pdf file
) (
html file
)
Purification of PCR Products (
pdf file
) (
html file
)
Cloning in Plasmid Vectors (
pdf file
) (
html file
)
Preparation of Sequencing Template for Plasmids (
pdf file
) (
html file
)
DNA Sequencing (
pdf file
) (
html file
)
PCR and Sequencing Primers Used in the Thomas Lab (
pdf file
) (
html file
)
Lab Stock Solutions
TRIS-ACETATE (TAE) (
pdf file
) (
html file
)
POTASSIUM PHOSPHATE BUFFER (for NGM plates) (
pdf file
) (
html file
)
LB BROTH, LB-TETRACYCLINE, LB AGAR, LB-TET AGAR (
pdf file
) (
html file
)
1.5% AGAR (for bacteria plates) (
pdf file
) (
html file
)
20% PEG 8000/2.5 M NaCl (for SPRI purification) (
pdf file
) (
html file
)
M9 BUFFER (
pdf file
) (
html file
)
NEMATODE FREEZING SOLUTION (
pdf file
) (
html file
)
Copyright © 2002-2008 UNH HCGS and the University of New Hampshire
Database design, development and management:
Fangning Liu
Funded by NSF
