The number in the primer name (i.e., 385F) corresponds to the approximate position of the 3´ nucleotide
of the primer based on the alignment; otherwise, primers are named the same as they were in
the paper or website referenced.
| Name | Sequence | Alignment/Reference |
|---|---|---|
| 18S-F-Mix | equal amounts of: G18S4, 18S-82F, EukF(10) and SSU_F_03 | |
| 18S-R-Mix | equal amounts of: EukR(10) and 18S-18P | |
| 18S-F-377 | TGC GCC TAC CAT GGT TGT AAC GGG T | MC174tr |
| 18s-f-447 | GGC TAC CAC ATC CAA GGA AGG CAG C | MC174tr |
| 18S-r-1650 | CAT CTA AGG GCA TCA CAG ACC TGT T | MC174tr |
| 18s-F-342 | GTC TGC CTT ATC AAC TTT CGA TGG | MC174tr |
| 18s-f-385 | CGG TGG TTA TAA CGG GTA ACG GAG | MC174tr |
| 18S-R-1837 | CTT ACT GGG AAT TCC TCG TTC AAG | MC174tr |
| SSU_F_11 | AAG TCT GGT GCC AGC AGC CGC | http://nema.cap.ed.ac.uk/biodiversity/sourhope/nemoprimers.html |
| 18S-R-530 | GCG GCT GCT GGC ACC AGA CTT | COMPLIMENT OF SSU_F_11 |
| SSU_F_02 | GGA AGG GCA CCA CCA GGA GTG G | http://nema.cap.ed.ac.uk/biodiversity/sourhope/nemoprimers.html |
| 18S_R_1108 | CCA CTC CTG GTG GTG CCC TTC C | COMPLIMENT OF SSU_F_02 |
| 18S-1410R | AAC YAA GAA CGG CCA TGC AYC AC | MC174tr |
| SSU-550F | GGC AAG TCT GGT GCC AGC AGC C | MC174tr (smilar to SSUF11) |
| 18S-G18S4 | GCTTGTCTCAAAGATTAAGCC | De Ley Lab |
| 18S-18P | TGATCCWKCYGCAGGTTCAC | De Ley Lab |
| 18S-82F | CTCAARGAYTAAGCCATGCA | Lopez-Garcia et al. 2002 |
| SSU_F_03 | GCTTGTCTCAAAGATTAAGCCATGC | http://nema.cap.ed.ac.uk/biodiversity/sourhope/nemoprimers.html |
| eukF(10) | AAC CTG GTT GAT CCT GCC AGT | Reference for this primer will be posted shortly |
| eukR(10) | TGA TCC TTC TGC AGG TTC ACC TAC | Reference for this primer will be posted shortly |