NEMATODE FREEZING SOLUTION

NaCl 5.85 g

KH₂PO₄ 6.8 g

Glycerol 300 g (1.25g/ml = 240 ml)

NaOH (1M) 5.6 ml

dH₂O 1 L

Autoclave 15 min liquid cycle, then add 3 ml MgSO₄ (.1 M).

Store at room temp.