PCR and sequencing primers used in the Thomas Lab

 

 

No.

Primer

Amplified gene

Sequence (5’-3’)

 

Reference

1

D2a

28S

ACAAGTACCGTGAGGGAAAGT

 

Nunn, 1992

2

D3b

28S

TGCGAAGGAACCAGCTACTA

 

 

3

Ferg-ID2B

28S

AGTAACCTCTTGCACCAAAC

 

 

4

D2B

28S

GACCCGTCTTGAAACACGGA

 

 

5

D3aR

28S

TCCGTGTTTCAAGACGGGTC

 

 

6

D3bR

28S

TAGTAGCTGGTTCCTTCGCA

 

 

7

D2F1-Ferg    

28S

 AGTACCGTGAGGGAAAGTTGAA        

 

Weimin

8

D2R-Ferg            

28S

GATAGTTCGATTAGTCTTTCGCCC      

 

Weimin

9

D2F2-Ferg

28S

GGAAAGTTGAAAAGCACTTTG

 

Weimin

10

COI-F1

Cytochrome oxidase subunit I

CCTACTATGATTGGTGGTTTTGGTAATTG

 

Kanzaki & Futai, 2002

11

COI-R2

Cytochrome oxidase subunit I

GTAGCAGCAGTAAAATAAGCACG

 

Kanzaki & Futai, 2002

12

COI-R1

Cytochrome oxidase subunit I

GTTGAGGGAAAAATGTTAAATTAACTCC

 

Kanzaki & Futai, 2002

13

COI-F2

Cytochrome oxidase subunit I

CCTGTCTTGGCTGGTGCTATTAC

 

Kanzaki & Futai, 2002

14

COI-F3

Cytochrome oxidase subunit I

TGAGCTCACCATATGTATACAGTAGG

 

Kanzaki & Futai, 2002

15

COI-R3

Cytochrome oxidase subunit I

CAATGTAGAGCAAAAATAACTAAATC

 

Kanzaki & Futai, 2002

16

CO1FD

Cytochrome oxidase subunit I

ATRGTNATRCCNACNATRATYGGNGG

 

Weimin

 17

CO1RD

Cytochrome oxidase subunit I

CCRGCRAARTGNARNGGRAARAANGT

 

Weimin

18

CO1InF

Cytochrome oxidase subunit I

ATGATTGGTGGTTTTGGTAA

 

Weimin

19

CO1InR

Cytochrome oxidase subunit I

ACAACCAATTAAACCAATTC

 

Weimin

20

18S-G18S4

18S

GCTTGTCTCAAAGATTAAGCC

 

De Ley et al., 2002

21

18S-18P

18S

TGATCCWKCYGCAGGTTCAC

 

De Ley et al., 2002

22

18S AR(420)

18S

CATCTAAGGGCATCACAGACCTGTT

 

Kelley

23

18S BR(440)

18S

CATCTAAGGGCATCACGGACCTGTT

 

Kelley

24

18S

18S

AAGTCTGGTGCCAGCAGCCGCGGT

 

Kelley

25

18SF-Burs

18S

ATGCATGTCTAAGTGGAGTATTATA

 

Weimin

26

18SR-Burs

18S

CTACGGCTACCTTGTTACGACTTTT

 

Weimin

27

M13R2,1 Topo

Topo TA cloning

GAAACAGCTATGACCATGATTACGCC

 

 

28

-47

Topo TA cloning

CGCCAGGGTTTTCCCAGTCACGAC

 

 

 

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